Lecture 6 sample preparation solid phase extraction exhaustive extraction analyte either adsorps or absorbs to a solid. But what are the options if you want to extract data from pdf documents. Figure 4 explodedviewof thespeedisc usedforsolid phase, extraction. In this sense, liquidliquid and solidphase extraction techniques should be considered complementaryapproaches,andalthoughthegeneral trend is towards the replacement of liquidliquid ex. Volatilization volatilization of a chemical from the surface of a. Figure 3 typical cartridge and vacuum filtration formats for solid phase extraction using discs. Phase extraction contaminated area sf 21,780 21,780 43,560 43,560 depth to groundwater ft 15 15 15 15 soil type sandsiltsiltysand mixture siltsiltyclay mixture sandsiltsiltysand mixture siltsiltyclay mixture flow per well gpm 5. Cost is low accuracy is greater glass breakage minimal lower solvent consumption reagent consumption lower no cross contamination less sample handling process in fewer steps. Solid phase extraction analysis applications library. Several extraction methods have been described to determine ocps in di. Mpe singlepump technology employs a single pump to extract fluids e. Extraction can be defined as the process of obtaining constituents by separating it from the crude material by the use of solvents. Apr 08, 2015 the sugar will move to the phase in which it is most soluble.
Solid phase extraction spe principle solid phase extraction spe is a sample preparation process by which compounds that are dissolved or suspended in a liquid mixture are separated from other compounds in the mixture according to their physical and chemical properties. Dualphase extraction dpe, also known as multiphase extraction, vacuumenhanced extraction, or sometimes bioslurping, is a technology that uses a high vacuum system to remove various combinations of contaminated ground water, separatephase petroleum product, and hydrocarbon vapor from the subsurface. Automated so lidphase extraction o triclosan in tap water using hydrophilic reversedphase cartridges and hplc with uv detection triclosan is a powerful antibacterial and antifungal agent that is an ingredient added to many consumer products to. Aqueous twophase extraction and purification of animal. Sample preparation columns based on the principles of solid phase extraction are fast becoming indispensable tools in many types of research. Some of them are discussed below so let us check it out one by one. Solidphase extraction spe is a technique designed for rapid sample preparation and purification prior chromatographic analysis. A free powerpoint ppt presentation displayed as a flash slide show on id. Powerpoint presentation on extraction of titanium titanium. Sve induces subsurface air flow by a vacuum applied to a sealed well screened in the zone of interest. It is also known as total fluids extraction or dualphase extraction. Jul 24, 2009 in this work, solid phase extraction spe trapping performance of lutein and betacarotene, which were used as the model molecules of carotenoids, was investigated. Dispersive solid phase extraction combined with dispersive liquid.
Volatilization volatilization of a chemical from the surface of a liquid is a partitioning. Solid phase extraction applications guide and bibliography a resource for sample preparation methods development, sixth edition editors. Mar 15, 2000 demonstrating the relationship of the basic theory of solid phase extraction spe to chromatography, this comprehensive reference illustrates how spe techniques significantly contribute to the preparation of samples for a wide variety of analytical techniques. If k d is equal to 1, equivalent concentrations exist in each phase. Solid phase extraction can be used to isolate analytes of interest. In order g003572for polar retention to occur between the sorbent and the sample, the analyte must be introduced to the spe device in a nonpolar sample or mobile phase environment. It is usually used to clean up a sample before using a chromatographic or other analytical method to quantitate the amount of analytes in the sample the general procedure is to load a solution onto the spe phase, wash away undesired. So let us find out some information to know more about it.
Theyll give your presentations a professional, memorable appearance the kind of sophisticated look that todays audiences expect. Solidphase extraction an overview sciencedirect topics. The sugar will move to the phase in which it is most soluble. Media in category solid phase extraction the following 7 files are in this category, out of 7 total.
Solid phase extraction is a simple form of liquid chromatography. Normal phase methodology in solid phase extraction microbiology. Solidphase extraction spe is an extraction method that uses a solid phase and a liquid phase to isolate one, or one type, of analyte from a solution. The technology is employed to facilitate mass removal of residual and vapor phase vocs located in the vadose zone. Solid phase extraction is one of the most commonly used methods for sample preparation. Israel artificial intelligence center sri international 333 ravenswood ave. To determine percent of fat content in a food sample by weight in a sample. Method 3535a solidphase extraction spe sw846 is not intended to be an analytical training manual. Retention mechanisms applied in solid phase extraction for some polar compounds elena bacalum, a. Extraction of the liquid extraction teknologi dan rekayasa kinetics of extraction it is important to investigate the rate at which the solute is transferred between the two phases, in some cases by an alteration of the contact time it is possible to alter the selectivity of the extraction. Solid phase microextraction was invented in 1990 by dr. Manually rekeying pdf data is often the first reflex but fails most of the time for a variety of reasons. Winner of the standing ovation award for best powerpoint templates from presentations magazine. In this work, solidphase extraction spe trapping performance of lutein and betacarotene, which were used as the model molecules of carotenoids, was investigated.
Dispersive solid phase extraction combined with dispersive liquidliquid extraction for the determination of btex in soil samples. Solid phase microextraction spme, is a solid phase extraction technique that involves the use of a fiber coated with an extracting phase, that can be a liquid or a solid, which extracts different kinds of analytes including both volatile and nonvolatile from different kinds of media, that can be in liquid or gas phase. Demonstrating the relationship of the basic theory of solidphase extraction spe to chromatography, this comprehensive reference illustrates how spe techniques significantly contribute to the preparation of samples for a wide variety of analytical techniques. Liquidliquid extraction lle, also known as solvent extraction and partitioning, is a method to separate compounds or metal complexes, based on their relative solubilities in two different immiscible liquids, usually water polar and an organic solvent nonpolar. A sample preparation primer and guide to solid phase. It is usually used to clean up a sample before using a chromatographic or other analytical method to quantitate the amount of analytes in the sample.
Check out advantages and disadvantages of debentures discussed below. Analytical laboratories use solid phase extraction to concentrate and purify samples for analysis. Solid phase extraction spe is an extraction method that uses a solid phase and a liquid phase to isolate one, or one type, of analyte from a solution. It is particularly useful for animal proteins, as it overcomes difficulties of other methods in removing bulk insoluble material, while at the same time providing purification with respect. View notes lecture 6 from chm 410 at university of toronto. Before a solid sample can be processed by spe, it must first be. Worlds best powerpoint templates crystalgraphics offers more powerpoint templates than anyone else in the world, with over 4 million to choose from. Chart and diagram slides for powerpoint beautifully designed chart and diagram s for powerpoint with visually stunning graphics and animation effects. Ppt batch extraction powerpoint presentation free to. Solid phase extraction spe became the most powerful trace enrichment method recently since. However, conventional extraction methods such as hydrophilic polymer. Solid phase extraction spe is an increasingly useful sample preparation technique. Multiphase extraction uses a vacuum system, which can also be combined with a downhole pump, to remove various combinations of contaminated groundwater, separatephase petroleum product, and vapors from the subsurface. Volume ratio was varied by selecting different total phase compositions along the selected tie line.
Figure 5 multiwell plate for automated solid phase extraction. The primary ways of extraction of organic molecules of interest to biologist and medical investigators involve breaking of the cells. Aqueous twophase systems provide a rapid, easily scalable method for separation of soluble proteins from insoluble materials and other undesired proteins. It provides stepbystep details on the applications of spe to environmental matrices, broadspectrum drug screening, veterinary drug. Solid phase extraction exhaustive extraction analyte either adsorps or absorbs to a solid support large concentrations possible limited use of solvents very. Conventional solid phase extraction is easy to automate both online and offline. Offered in a variety of chemistries and sizes, dionex solex spe cartridges trap and concentrate your analytes of interest from largevolume aqueous matrices. In the experiments reactive copper phthalocyanine dye attached to silanized magnetite, and magnetic charcoal were used as adsorbents and selected organic dyes were used as. With spe, many of the problems associated with liquidliquid extraction can be prevented, such as incomplete phase separations, lessthanquantitative recoveries, use of expe nsive, breakable specialty glassware, and disposal of large quantities of organic.
Menlo park, ca we have prepared a set of notes incorporating the visual aids used during the information extraction tutorial for the ijcai99 tutorial. The complete guide to solid phase extraction spe phenomenex. The system lowers the water table around the well, exposing more of the formation. Solid phase microextraction spme samantha keene tuesday, 30 june 2009 who discovered spme. Solidphase extraction spe is an extractive technique by which compounds that are dissolved or suspended in a liquid mixture are separated from other compounds in the mixture according to their physical and chemical properties.
It is also known as total fluids extraction or dual phase extraction. The phase volume ratio v r is defined as the ratio of volume of the top phase to that of the bottom phase albertsson, 1985. Solid phase extraction an overview sciencedirect topics. Normalphase methodology in solid phase extraction microbiology. Solid phase extraction of organochlorine pesticides with. Analytical laboratories use solid phase extraction to concentrate and.
With spe, many of the problems associated with liquidliquid extraction can. A solid phase extraction consists of bringing a liquid or gaseous test sample in contact with a solid phase, whereby the analyte is selectively adsorbed on the surface of the solid phase other solvents liquids or gasesadded to remove possible adsorbed matrix components eluting. You can optimize your spe protocols by using silicycle siliaprep and siliaprepx spe cartridges and well plates. The primary ways of extraction of organic molecules of interest to biologist and. An introduction to basic theory, method development, and applications conference paper pdf available november 2015 with 4,431 reads how we measure reads. In dpe systems a highvacuum extraction well is installed with its screened section in the zone of contaminated soils and groundwater. Reproduced with permission from plumb rs, gray rdm, and jones cm 1997. Aug 25, 2011 defination of solid phase extraction spe. This system automates spe for up to 6 samplesfrom cartridge or disk conditioning, sample loading, rinsing, and eluting steps, washing, and eluting. Powerpoint presentation on extraction of titanium free download as powerpoint presentation. Normalphase methodology in solid phase extraction sigma. D is large, indicating a high degree of extraction from phase a into phase b. Magnetic solidphase extraction is a new procedure for the preconcentration of target analytes from large volumes based on the use of magnetic or magnetizable adsorbents. To study the function of extraction process by soxhlet extractor.
Advantages and disadvantages of solid phase extraction. Our new crystalgraphics chart and diagram slides for powerpoint is a collection of over impressively designed datadriven chart and editable diagram s guaranteed to impress any audience. Magnetic solid phase extraction is a new procedure for the preconcentration of target analytes from large volumes based on the use of magnetic or magnetizable adsorbents. Solidphase extraction is a simple form of liquid chromatography. Sanderchemical sciences divisionmaterial measurement laboratorynational institute of standards and technologygaithersburg, md 20899solid phase extraction is a form of liquid chromatography used in processing samples to selectively isolate constituents of interest from other compounds that may interfere with the analysis. Lecture 6 lecture 6 sample preparation solid phase. In general, spe involves transference of the analytes from a liquid sample to a solid sorbent, usually followed by elution. Two basic mechanisms were applied for studying some polar compounds of pharmaceutical interest captopril, atenolol, and metformin by solid phase extraction, using an octadecilsilica based adsorbent. Conventional solidphase extraction is easy to automate both online and offline. C30, c18, diol, and silica, were compared respectively with the help. In todays work environment, pdf became ubiquitous as a digital replacement for paper and holds all kind of important business data. The absorption, elution, and enrichment of carotenoids on spe cartridges with four different sorbents, i. Automated solid phase extraction spe thermo fisher.
You may need a pdf reader to view some of the files on this page. Introduction to information extraction technology a tutorial prepared for ijcai99 by douglas e. Soil vapor extraction sve is the extraction of soil vapor from the semisaturated subsurface, or vadose zone. Multi phase extraction uses a vacuum system, which can also be combined with a downhole pump, to remove various combinations of contaminated groundwater, separate phase petroleum product, and vapors from the subsurface. There is a net transfer of one or more species from one liquid into another liquid phase, generally from aqueous to organic. Liquidliquid extraction is a classical method for preconcentrating metal ions andyor matrix removal. The debentures which are generally issued by the corporations, are promises to pay specific amount of principal or money at a specified time or date over the particular course of the loan tenure, during which time interest is paid at a fixed rate on decided dates.
Liquid liquid extraction and flocculation linkedin slideshare. Conversely, if k d is small, less chemical x is transferred from phase a into phase b. Solidphase extraction for liquid samples became a widely used laboratory technique with the introduction of disposable sorbent cartridges containing porous, siloxanebonded silica particles, sized to allow sample processing by gentle suction figure 2. Let us discuss advantages and disadvantages of solid phase extraction. Ppt batch extraction powerpoint presentation free to view. Solid phase extraction exhaustive extraction analyte either adsorps or absorbs to 964518 ppt. Therefore, method procedures are written based on the assumption that they will be performed by analysts who are formally trained in at least the basic principles of chemical analysis and in the use of the subject technology. Dualphase extraction dpe, also known as multiphase extraction, is a technology that uses a highvacuum system to remove both contaminated groundwater and soil vapor. A range of phases is commercially available, such as silica, c 18, c 8, c 5, c 2, phenyl, diol, amino bonded silica, ion exchange phases and polymer phases. A typical solidphase extraction cartridge consists of a short column generally an open syringe barrel containing a sorbent with a nominal.
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